Enzyme sensors, methods for preparing and using such sensors, and methods of detecting protease activity

摘要

An enzyme sensor comprising:
a) a molecular recognition motif that binds an enzyme; and
b) an optically-active fluorescent host protein in which the molecular recognition motif having an amino acid sequence is operatively linked to or integrated therein,
wherein the interaction of the analyte to the molecular recognition motif produces a detectable change,
characterized in that the molecular recognition motif comprises a sequence according to SEQ ID No: 1 a, wherein
X 1 is one amino acid and
X 2 is either non-existent, or one amino acid.