METHOD FOR ISOLATING NUCLEIC ACIDS FROM A VETERINARY WHOLE BLOOD SAMPLE

摘要

The present invention provides a method for isolating nucleic acids from a veterinary whole blood sample, said method comprising at least the following steps a) preparing a binding mixture comprising—the lysed sample—at least one chaotropic agent—at least one alcohol—at least one polyoxyethylene fatty alcohol ether; b) passing the binding mixture through a column comprising a nucleic acid binding solid phase thereby binding the nucleic acids to the nucleic acid binding solid phase; c) optionally washing the nucleic acids while being bound to the solid phase; d) optionally eluting the nucleic acids from the solid phase. It was found that the addition of the specific non-ionic detergent overcomes the problems of the prior art methods, wherein the column clogs what prevents the efficient nucleic acid isolation from this difficult sample. When the specific non-ionic detergent is included into the binding mixture, no clogging occurs thereby allowing the efficient isolation of nucleic acids from veterinary whole blood samples.