METHODS FOR CULTURING ENDOTHELIAL PROGENITOR CELLS DERIVED FROM HUMAN UMBILICAL CORD BLOODS AND COMPOSITIONS FOR PREVENTING OR TREATING ISCHEMIC DISEASES COMPRISING ENDOTHELIAL PROGENITOR CELLS DERIVED FROM HUMAN UMBILICAL CORD BLOODS

摘要

The present invention provides a method for culturing endothelial progenitor cells derived from human umbilical cord blood. According to the present invention, the endothelial progenitor cells derived from human umbilical cord blood cultured zare capable of having the cell adhesive ability and proliferation ratio similar to a cell cultured by a conventional cell culturing method. According to the present invention, collagen derived from umbilical cord and umbilical cord extract can be used as serum replacement and adjurvants for culturing a stem cell and a general animal cell. According to the present invention, a vascular endothelial (precursor) cell, and extract and collagen derived from umbilical cord, which are an autologous substance, are capable of preventing an immune rejection response generated when a cell is transplanted; and minimizing the pollution caused by a pollution source derived from an animal by excluding the use of a protein source derived from an animal. The present invention provides a pharmaceutical composition comprising endothelial progenitor cells derived from human umbilical cord blood for preventing or treating ischemic diseases.