MELTING CURVE ANALYSIS USING SELF INTERNAL CONTROL AND PNA PROBE COMPRISING REPORTER AND QUENCHING, METHOD AND KIT FOR ANALYZING TARGET DNA DETECTION USING MELTING CURVE ANALYSIS

摘要

The present invention relates to a method for analyzing a melting curve using a PNA probe with which an internal control, a reporter, and a quencher are combined, a method for detecting a target nucleic acid, and a kit for detecting a target nucleic acid, and more specifically, to a method for analyzing a melting curve using the melting temperature difference between a target nucleic acid and an internal control, which is characterized by substituting, deleting, or inserting one to four bases of a target nucleic acid base sequence for effectively detecting a target nucleic acid, and for distinguishing a false negative signal from a false positive signal of a PCR, and a method for detecting a target nucleic acid and a kit for detecting a target nucleic acid using the same. The present invention has an effect of distinguishing a false negative signal from a false positive signal, and effectively detecting a target nucleic acid through the melting curve analysis by using a PNA probe with which an internal control, which is characterized by substituting, deleting, or inserting one to four bases of a target nucleic acid base sequence, a reporter, and a quencher are combined.