Method of delivering RNA interference and uses thereof

摘要

The invention provides a method of RNA interference, which comprises contacting the cell with a fusion protein-double stranded RNA complex, the complex comprising the double stranded RNA segment containing a double stranded RNA of interest and a fusion protein, the fusion protein comprising (1) a targeting moiety, which will specifically binds to a site on a target cell, and (2) a binding moiety, which will bind to the double stranded RNA, wherein the double stranded RNA segment initiates RNA interference in the cell.

主权项

1. A composition that selectively binds to an antigen on the surface of a target cell and selectively initiates RNA interference in the cell comprising:
a) an siRNA molecule comprising a double stranded RNA segment of about 15-50 base pairs in length, wherein one of one strands is complementary and the other strand identical to a RNA interference target RNA; and b) a fusion protein, comprising (1) an antibody that binds specifically to an antigen on the surface of a target cell, and (2) a protamine protein, or fragment thereof, capable of binding to double stranded RNA without chemical conjugation.