Polymeric collagen biomaterials

摘要

This invention relates to the production of biomaterials from polymeric collagen (PC) and its seeding with cells and other components of biomaterials. Polymeric collagen is isolated from tissue, suspended in an acidic solution, and then neutralized at low temperature. The neutralized suspension is then seeded with biomaterial components, such as cells, and the polymeric collagen aggregated to form a biomaterial comprising the biomaterial components. Polymeric collagen biomaterials produced as described herein may therefore be useful in a range of tissue engineering applications.

主权项

1. A method of producing a polymeric collagen biomaterial Comprising:
(i) depleting calcium from a sample of collagen tissue, optionally a sample of tendon or ligament from a bird or a mammal, (ii) dispersing the calcium depleted tissue sample in an acidic solution to produce a tissue suspension, (iii) neutralising the tissue suspension and aggregating the polymeric collagen therein, (iv) removing aggregated polymeric collagen from the neutralised tissue suspension, (v) dispersing the aggregated polymeric collagen in an acidic solution to produce a polymeric collagen suspension, (vi) neutralising the polymeric collagen suspension and aggregating the polymeric collagen therein, (vii) removing aggregated polymeric collagen from the neutralised suspension, (viii) performing one or more repetitions of steps (v) to (vii), (ix) dispersing the aggregated polymeric collagen in an acidic solution to produce a purified polymeric collagen suspension, (x) neutralising the purified polymeric collagen suspension at 0 to IO° C. without aggregation of the polymeric collagen therein, (xi) incorporating one or more biomaterial components into the neutralised suspension, and; (xii) aggregating the polymeric collagen in the suspension resulted from the step (xi) to form said polymeric collagen biomaterial.