发明名称 Culture medium and method for inducing differentiation into adipocytes
摘要 Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum.
申请公布号 US8785199(B2) 申请公布日期 2014.07.22
申请号 US201013202720 申请日期 2010.02.18
申请人 Fujirebio, Inc 发明人 Hotta Yoshiyuki
分类号 C12N5/00;C12N5/077 主分类号 C12N5/00
代理机构 Birch, Stewart, Kolasch & Birch, LLP 代理人 Birch, Stewart, Kolasch & Birch, LLP
主权项 1. A culture medium for inducing differentiation of mammalian somatic stem cells into adipocytes, comprising a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free.
地址 Tokyo JP