发明名称 |
Culture medium and method for inducing differentiation into adipocytes |
摘要 |
Disclosed are a culture medium, an additive, and a method for efficiently inducing the differentiation of mammalian somatic stem cells into cells having the characteristics of adipocytes under conditions of serum-free or low-serum culture medium. The culture medium for inducing the differentiation of mammalian somatic stem cells into adipocytes comprises a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free or contains a low concentration of serum. |
申请公布号 |
US8785199(B2) |
申请公布日期 |
2014.07.22 |
申请号 |
US201013202720 |
申请日期 |
2010.02.18 |
申请人 |
Fujirebio, Inc |
发明人 |
Hotta Yoshiyuki |
分类号 |
C12N5/00;C12N5/077 |
主分类号 |
C12N5/00 |
代理机构 |
Birch, Stewart, Kolasch & Birch, LLP |
代理人 |
Birch, Stewart, Kolasch & Birch, LLP |
主权项 |
1. A culture medium for inducing differentiation of mammalian somatic stem cells into adipocytes, comprising a basal medium for culturing mammalian cells, an agent for inducing the differentiation of mammalian somatic stem cells into adipocytes, biotin, a ligand for endothelial cell differentiation gene (Edg) family receptors, vitamin C, and HEPES, which culture medium is serum-free. |
地址 |
Tokyo JP |