Biochemical reactor

摘要

A biochemical reactor involves an online cell examination microscope thereon. The online cell examination microscope comprises a main body, an objective lens, an observation entrance window, a sampling device, an external light source system. Said observation entrance window lies in front of said main body and the sampling device lies in front of said observation entrance window. An objective lens and said external light source system lie behind the observation entrance window in the main body. A reflector prism is installed between the external light source system and the objective lens. A reflector lies behind the objective lens and in front of the observation entrance window. An annular diaphragm is placed in front of the reflecting prism. A CCD or an area image sensor is arranged on the upper side of the reflectance prism.

主权项

1. A biochemical reactor, wherein the biochemical reactor is equipped with an in-situ cell examination microscope,
wherein the in-situ cell examination microscope comprises a main body of the microscope, an object lens, an observation entrance window, a sampling device and an exterior light source system, wherein the observation entrance window is arranged in front of the main body of the microscope, the sampling device is arranged in front of the observation entrance window, and an object lens and an exterior light source system are both arranged behind the observation entrance window in the main body of the microscope, wherein a reflecting prism is arranged between the exterior light source system and the object lens, a reflector is arranged behind the object lens while in front of the observation entrance window, an annular diaphragm plate is arranged in front of the reflecting prism, and a CCD or an area array image sensor is arranged above one side of the reflecting prism; and wherein the sampling device consists of a sampling piece, a flexible element and a movable device, wherein the sampling piece and a driving axle of the movable device are connected by the flexible element, and the space between the front end face of the sampling piece and the observation entrance window forms a sampling pool; wherein a living cell sensor is further arranged on a body of the biochemical reactor, and wherein the biochemical reactor is capable of measuring physiological metabolic parameters to optimize and scale up a fermentation process, and wherein the flexible element is compressible or stretchable to adjust a distance between the front end face of the sampling piece and the observation entrance window.