Sugarcane bacilliform viral (SCBV) enhancer and its use in plant functional genomics

摘要

Identification of new enhancer sequence has significant utility in the plant functional genomics. The sugarcane bacilliform badnavirus (SCBV) transcriptional enhancer has been identified. This enhancer can be used to increase the rate of transcription from gene promoters and in activation tagging experiments. A ten-fold increase in transcription was observed when a 4× array of the SCBV enhancer was placed upstream of a truncated form of the maize alcohol dehydrogenase minimal promoter. Methods of using the SCBV transcriptional enhancer are described, as are chimeric transcription regulatory regions, constructs, cells, tissues, and organisms that comprise one or more copies of the enhancer.

主权项

1. A chimeric transcription regulatory region comprising:
a sugarcane bacilliform viral (SCBV) enhancer element consisting of position 337 to position 618 of SEQ ID NO: 1; and operably linked thereto, a heterologous promoter comprising an RNA polymerase binding site and a mRNA initiation site, wherein the chimeric transcription regulatory region enhances transcription of an operably linked nucleotide sequence of interest, compared to transcription of the nucleotide sequence of interest operably linked to a chimeric transcription regulatory region comprising the promoter and not comprising the SCBV enhancer sequence.