| 发明名称 | Detection of nicotine metabolites | ||
| 摘要 | The present invention relates to assays for detection of nicotine metabolites, in particular cotinine, in fluid samples and uses of these assays in quantification of smoking habits. The assays comprise contacting a body fluid sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound which is associated with the presence of nicotine metabolites. Also provided are assay kits including a cyanogen halide or cyanogen halide-precursor(s) and a pyrazolone compound. | ||
| 申请公布号 | US8785205(B2) | 申请公布日期 | 2014.07.22 |
| 申请号 | US201113697446 | 申请日期 | 2011.05.13 |
| 申请人 | GFC Diagnostics Ltd. | 发明人 | Knill Charles John;Kennedy John Frederick |
| 分类号 | G01N33/52;G01N33/50;G01N33/00;G01N33/94 | 主分类号 | G01N33/52 |
| 代理机构 | Myers Bigel Sibley & Sajovec, P.A. | 代理人 | Myers Bigel Sibley & Sajovec, P.A. |
| 主权项 | 1. A method of detecting or measuring a nicotine metabolite in a sample comprising contacting the sample with a cyanogen halide and a pyrazolone compound and detecting a change in light absorbance of the pyrazolone compound; wherein the pyrazolone compound is a compound according to formula Iin which: R1 is —C(═O)—; R2 is —CH2—; R3 is —C(R6)—; R5 is phenyl substituted with —S(═O)2OH; R6 is C1-6alkyl; z is a single bond and a is 1; and y is a double bond and b is 0. | ||
| 地址 | Oxon GB | ||