Method for producing L-amino acids using bacteria of the enterobacteriaceae family

摘要

There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.

主权项

1. A method for producing L-amino acid, which comprises cultivating a bacterium of Enterobacteriaceae family in a culture medium to cause accumulation of the L-amino acid in the culture medium, and isolating the L-amino acid from the culture medium, wherein said bacterium has been modified to enhance an activity of D-xylose permease and activities of xylose utilization proteins encoded by xylABFGHR locus by a method selected from the group consisting of:
a) increasing the copy number of a gene encoding D-xylose permease and the xylABFGHR locus, b) placing the gene encoding D-xylose permease and the xylABFGHR locus under the control of a potent promoter, and c) combinations thereof, wherein the carbon source in said culture medium consists of glucose; and wherein said D-xylose permease and said xylose utilization proteins encoded by xylABFGHR locus are both derived from a bacteria of the Enterobacteriaceae family.