Methods using pores

摘要

The invention relates to a method of identifying an individual nucleotide, comprising (a) contacting the nucleotide with a transmembrane protein pore so that the nucleotide interacts with the pore and (b) measuring the current passing through the pore during the interaction and thereby determining the identity of the nucleotide. The invention also relates to a method of sequencing nucleic acid sequences and kits related thereto.

主权项

1. A method of distinguishing an individual monophosphate nucleotide, comprising:
(a) contacting the monophosphate nucleotide with a transmembrane protein pore so that the monophosphate nucleotide interacts with the pore, wherein the pore comprises a molecular adaptor that facilitates the interaction between the monophosphate nucleotide and the pore, wherein the molecular adaptor is a cyclodextrin; and (b) measuring a current passing through the pore during the interaction and thereby distinguishing the monophosphate nucleotide from other monophosphate nucleotides.