| 摘要 |
FIELD: medicine.SUBSTANCE: group of inventions relates to biotechnology. A method for detecting an expression level of MCP1 (CCL2) gene provides assessing its iRNA amount by a polymerase chain reaction with reverse transcription (RT-PCR) with real-time signal detection in human sampled cells, tissues and biological fluids. What is developed is a kit for quantitation of iRNA of MCP1 involving combinations of oligonucleotide primers and fluorescent probes for real-time RT-PCR to detect an amount of cDNA of MCP1 genes and RPL32, ACTB, PII normalisation genes.EFFECT: using the group of inventions enables reducing calculation errors introduced when using one normalisation gene without increasing the number of amplifications, as well as reducing calculation errors raised in a simplified suggestion of the efficacy balance of the cDNA amplification reactions of MCP1 and a normalisation gene observed in ??Ct method.2 cl, 2 dwg, 3 tbl, 1 ex |
