| 发明名称 |
METHODS FOR INCREASING THE EFFICIENCY OF HYBRIDOMA GENERATION |
| 摘要 |
Disclosed herein are methods for improving the efficiency of the production of hybridomas, e.g., through enrichment of IgG expressing B cells. Also disclosed are hybridoma compositions produced using these methods as well as methods for producing antibodies with the hybridomas. |
| 申请公布号 |
US2014199319(A1) |
申请公布日期 |
2014.07.17 |
| 申请号 |
US201314106116 |
申请日期 |
2013.12.13 |
| 申请人 |
AbbVie, Inc. |
发明人 |
SEAGAL Jane;BARLOW Eve H.;HSIEH Chung-Ming;PAN Jeffrey Yen;JENNINGS Shawn M.;LEDDY Mary R.;THAKUR Archana B. |
| 分类号 |
C12N5/16;G01N33/569;C07K16/00;C12N15/02 |
主分类号 |
C12N5/16 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of generating a hybridoma comprising
a) providing spleen or lymph node cells from an animal and myeloma cells; b) isolating IgM negative B cells from the spleen or lymph node cells; and c) fusing the isolated IgM negative B cells and the myeloma cells;thereby generating a hybridoma. |
| 地址 |
Worcester MA US |
