RECOMBINANT ANTIBODY STRUCTURES BINDING TO AND BLOCKING THE ACTIVITY OF VASCULAR ENDOTHELIAL GROWTH FACTOR 2 (VEGFR- 2/KDR)

摘要

Phage displayed recombinant antibody library was developed and the library was screened against VEGFR-2. After screening and ELISA experiments two recombinant antibodies showing binding properties to VEGFR-2 was obtained. The difference of the two recombinant antibodies to the recombinant antibodies already developed was demonstrated by DNA sequence analysis. The inhibition effect of the two recombinant antibodies on endothelial cell proliferation was demonstrated with cell assays. Thus these recombinant antibodies might be used as VEGF related angiogenesis inhibitors.

主权项

1. A recombinant antibody selection method based on phage display technology that inhibits intracellular signal transduction and proliferation in the cells that express VEGF receptor 2 (VEGFR-2), characterized by obtaining the recombinant antibodies which block VEGFR-2 activity through a recombinant antibody library.