KIT AND METHOD FOR THE CAPTURE OF TUMOR CELLS

摘要

The invention relates to a kit and method for the capture of tumor cells in a body fluid sample. The kit and method of the invention can capture living tumor cells but not non-living tumor cells or cell fragments so that the tumor species can be further identified by further culture of the captured tumor cells. Also, the kit and method of the invention can readily identify whether a sample contains tumor cells and collect these tumor cells for further identification so that the presence of cancer and development of the metastasis and early relapse can be found.

主权项

1. A method for the capture of circulating tumor cells in a body fluid sample or a serum-containing sample, comprising the following steps:
(a) attaching endothelium or epithelium cells on a solid support, (b) inducing an inflammatory reaction of the cells of (a) with one or more inflammation-inducing agent, wherein the inflammation-inducing agent is tumor necrosis factor or interleukin; (c) adding white blood cells so that they attach on the cells of (a), wherein the white blood cells are neutrophilis, eosinophils, basophils, lymphocytes, monocytes or macrophages; and (d) adding a body fluid sample or a serum containing sample to the solid support whereby the white blood cells and the endothelium or epithelium cells capture tumor cells contained in the body fluid sample or the serum-containing sample, wherein the body fluid sample or the serum-containing sample is pre-diluted more than 10 times.