METHOD FOR DETERMINING THE AMOUNT AND/OR ACTIVITY OF ANTIOXIDANTS

摘要

The invention relates to a method of determining amount and/or activity of antioxidants in test samples, wherein a reaction mixture with known initial concentrations of a source of free radicals and a chemiluminescence activator is to measured photometrically starting from the instant of reaction initiation, a reaction rate constant (kR) for generating free radicals and a reaction rate constant (kLum) for eliminating free radicals upon interacting with the activator are calculated based on dependence of light radiation intensity of the mixture on the time of reaction; a new reaction mixture of the above-mentioned source and activator with known initial concentrations and containing antioxidant is prepared; said mixture is measured photometrically; and an initial concentration of antioxidant [In]0 and a reaction rate constant for interaction between free radicals and the antioxidant (kIn) are calculated, based on the change in light radiation intensity in relation to the time of reaction.

主权项

1. A method of determining amount and/or activity of an antioxidant in a test sample, the method including:
performing photometric measurements of a reaction mixture having known initial concentrations of a source of free radicals ([AR]0) and a chemiluminescence activator ([Lum]0), starting from the instant of reaction initiation; calculating a reaction rate constant (kR) for generating free radicals and a reaction rate constant (kLum) for eliminating free radicals upon interacting with the activator, based on dependence of light radiation intensity of the mixture on the time of reaction; preparing a new reaction mixture having known values of [AR]0 and [Lum]0 and containing an antioxidant; performing photometric measurements of the new mixture, taking into account the known values of [AR]0, [Lum]0, kR, and kLum, based on change in chemiluminescence intensity of the new reaction mixture, depending on duration of the period of time passing from the instant of reaction initiation; and calculating initial concentration [In]0 of the antioxidant and a reaction rate constant (kIn) for interaction between free radicals and the antioxidant.