METHODS FOR MEASURING THE METABOLISM OF NEURALLY DERIVED BIOMOLECULES IN VIVO

摘要

The present invention relates to methods of diagnosing, monitoring, and assessing treatment effects for neurological and neurodegenerative diseases and disorders, such as Alzheimer's Disease, early in the course of clinical disease or prior to the onset of brain damage and clinical symptoms. Methods of measuring the in vivo metabolism of biomolecules produced in the CNS in a subject are provided.

主权项

1. A method for measuring the in vivo metabolism of a protein in a subject, the protein being synthesized in the central nervous system, the method comprising:
(a) administering a labeled moiety to the subject, the labeled moiety being capable of crossing the blood brain barrier and incorporating into the protein as the protein is synthesized in the central nervous system of the subject; (b) obtaining a biological sample from the subject, the biological sample comprising a protein fraction labeled with the moiety and a protein fraction not labeled with the moiety; and (c) detecting the amount of labeled protein and the amount of unlabeled protein, wherein the ratio of labeled protein to unlabeled protein is directly proportional to the metabolism of the protein in the subject.