| 摘要 |
A method for detecting a sentinel lymph node generating a microenvironment suitable for micrometastasis within the sentinel lymph node of carcinoma cells from a primary tumor or for detecting micrometastasis within the sentinel lymph node. A detecting agent is injected into the primary tumor, or tissue or a lymph node nearby the tumor, which allows the detecting agent to reach the sentinel lymph node by lymphatic circulation. The detecting agent includes colloid particles, an anti-ICAM-1 antibody or an ICAM-1 ligand exposed on an outer surface of the colloid particles, and a detectable label. Positive detection of the detectable label within the lymph vessel or the sentinel lymph node indicates that ICAM-1 is present, and the presence of ICAM-1 indicates that the sentinel lymph node is generating a microenvironment suitable for micrometastasis within the sentinel lymph node of carcinoma cells from the primary tumor or that micrometastasis is occurring within the lymph node. |
| 主权项 |
1. A method for detecting a sentinel lymph node generating a microenvironment suitable for micrometastasis within the sentinel lymph node of carcinoma cells from a primary tumor or for detecting micrometastasis within the sentinel lymph node, the method comprising:
injecting a detecting agent into the primary tumor, or tissue or a lymph node nearby the tumor, which allows the detecting agent to reach the sentinel lymph node by lymphatic circulation, the detecting agent comprising:
colloid particles selected from the group consisting of micelle particles of a biodegradable resin, micelle particle of a synthetic resin, liposomes, and serum albumin;a detectable label comprising at least one of a fluorescent agent, a contrast agent, and a chemokine to which an infrared-chromogenic dye is bonded; andan anti-ICAM-1 antibody or an ICAM-1 ligand exposed on an outer surface of the colloid particles, the ICAM-1 ligand being selected from the group consisting of CD11a, CD11b, and CD11c;wherein the detectable label is:
contained within the colloid particles,attached to the colloid particles, orattached to the anti-ICAM-1 antibody; allowing the anti-ICAM-1 antibody or the ICAM-1 ligand of the detecting agent to bond to ICAM-1 present on an endothelial cell of either a lymph vessel or the sentinel lymph node that is downstream of the primary tumor; and detecting and determining the location of the detectable label; wherein positive detection of the detectable label within the lymph vessel or the sentinel lymph node indicates that ICAM-1 is present, and the presence of ICAM-1 indicates that the sentinel lymph node is generating a microenvironment suitable for micrometastasis within the sentinel lymph node of carcinoma cells from the primary tumor or that micrometastasis is occurring within the sentinel lymph node. |
