PRODUCTION OF MOTIF-SPECIFIC AND CONTEXT-INDEPENDENT ANTIBODIES USING PEPTIDE LIBRARIES AS ANTIGENS

摘要

PROBLEM TO BE SOLVED: To provide a production method of motif-specific, context-independent antibodies which are specific to one fixed amino acid residue of a peptide sequence.SOLUTION: In the context of variable surrounding amino acid or peptide sequences, the method comprises (1) the step of constructing a peptide library featuring at least modified or unmodified one fixed amino acid and variable surrounding amino acids, and (2) the step of immunizing a host with this peptide library. Antibodies may optionally selectively be isolated and purified from an antisera of this immunized host. The method comprises modified motifs, such as phosphothreonine, phosphoserine, MAPK consensus recognition sites, 14-3-3 consensus recognition sites, CDK consensus recognition sites, and acetylated-lysine, as well as unmodified motifs.