Combined tandem mass spectrometry and ion mobility mass spectrometry

摘要

A method of analysing ions comprising: performing an initial multi dimensional survey scan comprising separating parent ions according to a first physico­chemical property and separating the parent ions according to a second physico-chemical property; at least one parent ion of interest from the initial multi-dimensional survey scan is determined, the at least one parent ion of interest having a first value or range of the first physico-chemical property and a second value or range of the second physico-chemical property; and then, during a subsequent single cycle of separation, the method further comprises: separating parent ions according to the first physico-chemical property; where if the parent ions have a value or range of the first physico-chemical property which corresponds with the first value or range, then the method further comprises selecting parent ions of interest having a value or range of the first physico-chemical property which corresponds with the first value or range and which also have a value or range of the second physico­chemical property which corresponds with the second value or range; and where if the parent ions have a value or range of the first physico-chemical property which does not correspond with the first value or range then the method further comprises separating the parent ions according to the first physico-chemical property and separating the parent ions according to the second physico-chemical property. Also disclosed is a method of analysing ions as above, where after determining the at least one parent ion of interest from the initial multi-dimensional survey scan, the parent ions according to the first physico-chemical property are separated and fragmented or reacted to produced fragment or product ions, which are analysed. Further disclosed are analytical instruments arranged and adapted to perform the above methods.