Polyepitopic protein fragments of the HPV virus proteins E6 and E7. production and uses thereof in vaccination

摘要

Polyepitopic fragments (I) from the Nef protein of human immune deficiency virus (HIV), are new. Polyepitopic fragments (I) from the Nef protein of human immune deficiency virus (HIV), are new. (I) is contained within any of the sequences: PheProValThrProGluValProLeuArgProMetThrTyrLysAlaAlaValAspLeuSer- HisPheLeuLysGluLysGlyGlyLeu; ArgArgGlnAspIleLeuAspLeuTrpIleTyrHisThrGlnGlyTyrPheProAspTrpGln- AsnTyrThrProGlyProGlyValArgTyrProLeuThrPheGlyTrpCysTyrLysLeu; ValLeuGluTrpArgPheAspSerArgLeuAlaPheHisHisValAlaArgGluLeuHisPro- GluTyr. These represent, respectively, amino acids 68-97, 105-145 or 180-202 of Nef, and bind stably to, respectively, the human leukocyte antigen (HLA) type molecules A2, A3, A11, B7, B8, B14, B35, B62 or Cw8; A1, A2, A24, A32, B7, B18, B27, B35, B49, B57/58 or B62; or A1, A2, A3, B8, B35, or B52. Alternatively, (I) comprises their derivatives which bind to the same HLA molecules as the parent compounds, formed by substitution, deletion and/or addition of one or more amino acids, modification of at least one peptide bond (particularly replacement by retro or retro-inverso bonds) and/or substitution of at least one amino acid by a non-proteinogenic amino acid. Independent claims are also included for the following: (1) a nucleic acid (II) that encodes (I) or its derivatives; (2) mono- or poly-clonal antibodies (Ab) directed against (I) or its derivatives; and (3) pharmaceutical composition or vaccine containing at least one (I), or its derivative, (II) or Ab. ACTIVITY : Antiviral. No biological data is given. MECHANISM OF ACTION : (I) induce a specific immune response; particularly they induce cytolysis, by cytotoxic T cells (CTL), of cells that express (I) associated with appropriate HLA molecules and induce secretion of cytokines (particularly interleukins -2 and -4, and gamma -interferon) by these CTL; vaccine.