| 摘要 |
Animal feeds containing growth-promoting supplements, particularly Vitamin B12, are made by inoculating a medium of starch and protein-containing material of vegetable origin, e.g. a cereal, rice or wheat bran, peanut husk or corn lees, wetted with corn steep liquor, with a mould of the Neurospora or Rhizopus genus, spreading the medium over a support which permits aeration, allowing the mould to grow for some days, and then drying and pulverizing the dried product. For example, wheat bran moistened with corn steep liquor is inoculated with an agar culture of Neurospora crassa or Rhizopus nigricans, incubated at 32-36 DEG C. for some days, and spread over a layer, 5-10 cm. thick, of a mixture of wheat bran, cornflour, bean flour, starch lees, rice bran and corn steep liquor which has been sterilized by steam, and after 3-5 days, the mixture is dried at between 30 DEG and 120 DEG C.; alternatively, the mixture spread with a bran culture of N. crassa is, after 1-4 days, spread with a bran culture of R. nigricans, and 2-4 days more allowed before the drying is undertaken. |
