Methods for diagnosis, prognosis and methods of treatment

摘要

The present invention provides an approach for the determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, expression markers and other criteria, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of modulators of cellular activation allows for characterization of pathways and cell populations. Several exemplary diseases that can be analyzed using the invention include AML, MDS, and MPN.

主权项

1. A kit comprising
a) at least two modulators selected from the group consisting of Etoposide, Mylotarg, Daunorubicin, AraC, SCF, SDF1a, LPS, Thapsigargin, cyclosporine A, decitibine, azacitadine, Vorinostat, valproic acid, bortezomib, and clofarabine; and b) at least three binding elements specific to a compound selected from the group consisting of p-Slp-76, p-Plcg2, p-Creb, cleaved Parp, p-Chk2, p-p65/Rel-A, p-S6, Cleaved Caspase 3, Cleaved Caspase 8, Cytoplasmic Cytochrome C, p-H2AX, p-4EBP-1, and p21.