<i>Mycoplasma genitalium </i>detection assay based on the Mg219 gene

摘要

There is provided a method for detecting M. genitalium nucleic acid in a sample, comprising: (i) amplifying a nucleic acid sequence comprising a fragment of SEQ ID NO: 1 (Mg219 gene); and (ii) detecting said amplified nucleic acid sequence.

主权项

1. A method for specifically detecting M. genitalium nucleic acid including clinical isolate M. genitalium nucleic acid in a clinical sample, comprising:
(a) amplifying a nucleic acid sequence comprising a fragment of SEQ ID NO: 1, wherein said fragment of SEQ ID NO: 1 is at least 25 consecutive nucleotides of SEQ ID NO: 1, by contacting said sample with forward and reverse primers, to produce the amplified nucleic acid sequence; wherein said forward primer binds to a target site located between nucleotide residue 41 and nucleotide residue 125 of the nucleic acid strand complementary to SEQ ID NO: 48; and wherein said reverse primer binds to a target site located between nucleotide residue 197 and nucleotide 350 of SEQ ID NO: 48; (b) detecting the amplified nucleic acid sequence; and (c) detecting specifically M. genitalium nucleic acid including M. genitalium nucleic acid from clinical isolates in the clinical sample, based on the presence of said amplified nucleic acid sequence.