Floral dip method for transformation of camelina

摘要

The present invention provides methods for transforming Camelina plants. In particular, the present invention relates to transforming Camelina sativa plants through contacting the plants to a dipping solution comprising Agrobacterium, a sugar, and a nonionic surfactant. The methods do not require a vacuum filtration step. The present invention provides, for example, useful methods for developing transformation systems for Camelina sativa that can enable manipulation of its agronomic qualities.

主权项

1. A method of transforming a Camelina plant comprising within a first dipping cycle,
i) contacting the Camelina plant with a first transformation dipping solution comprising a sugar, a nonionic surfactant, and an Agrobacterium comprising a first expression vector capable of expression in the Camelina plant, wherein the contacting step comprises dipping the Camelina plant into the solution; ii) removing the plant from the first transformation dipping solution, thereby conducting a first contacting step to produce a first dipped plant; iii) incubating the first dipped plant; iv) in the same first dipping cycle, contacting the first dipped plant with a second transformation dipping solution comprising a sugar, a nonionic surfactant, and an Agrobacterium comprising a second expression vector capable of expression in the Camelina plant, thereby conducting a second contacting step to produce a twice dipped plant, wherein the contacting step comprises dipping the Camelina plant into the solution; v) removing the twice dipped plant from the second transformation dipping solution; and vi) incubating the twice dipped plant; wherein the sugar is a disaccharide, the nonionic surfactant is trisiloxane, and both flowers and buds are present during the dipping steps; wherein said method does not involve vacuum infiltration.