发明名称 |
Recombinant bacteria for producing deoxyviolacein and uses thereof |
摘要 |
Recombinant bacteria for producing deoxyviolacein and uses thereof are provided, wherein the recombinant bacteria is obtained by introducing the deoxyviolacein synthesis-related gene cluster into Escherichia coli BL21-CodonPlus (DE3)-RIL or Pseudomonas putida mt-2. The deoxyviolacein synthesis-related gene cluster is obtained by knocking out VioD gene from the violacein synthesis-related gene cluster composed of VioA, VioB, VioC, VioD and VioE, and the nucleotide sequence is as shown in the SEQ ID NO: 1 in the sequence listing. A method for producing deoxyviolacein by fermenting the recombinant bacteria to produce deoxyviolacein by using L-tryptophan as substrate is provided. The method has high efficiency of deoxyviolacein production, the deoxyviolacein produced is convenient to be extracted, and simple to be separated and purified. |
申请公布号 |
US8778654(B2) |
申请公布日期 |
2014.07.15 |
申请号 |
US200913003227 |
申请日期 |
2009.04.22 |
申请人 |
Tsinghua University |
发明人 |
Xing Xinhui;Jiang Peixia |
分类号 |
C07H21/00;C12N1/21;C12N15/00;C12P17/10;C12P21/00 |
主分类号 |
C07H21/00 |
代理机构 |
Alleman Hall McCoy Russell & Tuttle LLP |
代理人 |
Alleman Hall McCoy Russell & Tuttle LLP |
主权项 |
1. An isolated deoxyviolacein synthesis-related gene cluster comprising
a polynucleotide comprising SEQ ID NO: 1. |
地址 |
Bejing CN |