| 发明名称 |
Optical based delivery of exogenous molecules to cells |
| 摘要 |
A method of delivering exogenous molecules, comprising: providing a plurality of cells having a cell membrane; adding a plurality of exogenous molecules to the cells; exposing the cells to a defocused infrared (IR) light to permeabilize the cell membrane of the cells; and delivering the exogenous molecules to the cells through the permeablized cell membrane, wherein an intensity of the IR light at the optical focus is at least greater than or equal to an order of 104 W/cm2. |
| 申请公布号 |
US8778682(B2) |
申请公布日期 |
2014.07.15 |
| 申请号 |
US201113221161 |
申请日期 |
2011.08.30 |
| 申请人 |
General Electric Company |
发明人 |
Neculaes Vasile Bogdan;Conway Kenneth Roger;Garner Allen Lawrence;Loghin Evelina Roxana;Yazdanfar Siavash;Dylov Dmitry Vladimirovich;Davis Brian Michael;Joo Chulmin |
| 分类号 |
C12N15/87;C12N13/00 |
主分类号 |
C12N15/87 |
| 代理机构 |
|
代理人 |
Haeckl Jenifer E. |
| 主权项 |
1. A method of delivering exogenous molecules, comprising:
providing a plurality of cells having a cell membrane; adding a plurality of exogenous molecules to the cells; exposing the cells to an infra-red (IR) light to permeabilize the cell membrane for delivering the exogenous molecules to the cells through the permeablized cell membrane, wherein an average power density of the IR light at the optical focus is greater than or equal to an order of 104 W/cm2, and a peak power density is greater than or equal to an order of 108 W/cm2, and wherein an area of irradiation of the IR light is in a range from about 50 to 200 μm2. |
| 地址 |
Niskayuna NY US |
