发明名称 Culture method related to differentiation of pluripotent stem cells into blood cells
摘要 It is an object of the present invention to provide a method for efficient differentiation into blood cells. Specifically, in the present invention, when blood cells are produced from pluripotent stem cells such as ES cells or iPS cells in vitro, the cells are cultured under a low oxygen partial pressure to increase the efficiency of differentiating the pluripotent stem cells into hematopoietic progenitor cells, erythroid progenitor cells, and the like, so as to increase the number of finally obtained, desired blood cells.
申请公布号 US8778681(B2) 申请公布日期 2014.07.15
申请号 US201313790307 申请日期 2013.03.08
申请人 University of Tokyo;Terumo Kabushiki Kaisha 发明人 Sano Shinya;Eto Koji;Takayama Naoya;Nakauchi Hiromitsu
分类号 C12N5/00 主分类号 C12N5/00
代理机构 Pearne & Gordon LLP 代理人 Pearne & Gordon LLP
主权项 1. A method for producing hematopoietic progenitor cells or erythroid progenitor cells, which comprises culturing cells selected from the group consisting of pluripotent stem cells and cells at a differentiation stage between pluripotent stem cells and hematopoietic progenitor cells, and increasing an oxygen partial pressure from a low oxygen partial pressure of up to 10% to a higher oxygen partial pressure of at least 11% during said culturing of said cells, thereby producing the hematopoietic progenitor cells or the erythroid progenitor cells, wherein the low oxygen partial pressure is increased to the higher oxygen partial pressure when an indicator gene is expressed that indicates expression of a differentiation induction and the gene is selected from the group consisting of mesendoderm induction, hematopoietic progenitor cell induction, differential proliferative cytokine, and blood cell induction.
地址 Tokyo JP