发明名称 |
IMPEDANCE-BASED SENSING OF ADHERENT CELLS ON A DIGITAL MICROFLUIDIC DEVICE |
摘要 |
Devices and methods are provided for measuring, on a digital microfluidic platform, electrical signals associated with the impedance of adherent cells. In one embodiment, a sub-droplet of cell culture media containing adherent cells is passively dispensed at a pre-selected electrode location where a local hydrophilic surface region is provided, and adherent cells are attached to the local hydrophilic surface region. The cell culture media sub-droplet is replaced with a sub-droplet of a low-conductivity medium in a passive dispensing step, retaining the attached adherent cells. An AC voltage with a suitable frequency is applied between electrodes of the device and a signal associated with the impedance of the adherent cells is obtained. One of the electrodes to which the AC voltage is applied may be a dedicated sensing electrode. The local thickness of a dielectric layer coating the pre-selected electrode may be reduced to increase the detection sensitivity of the device. |
申请公布号 |
CA2830810(A1) |
申请公布日期 |
2014.07.14 |
申请号 |
CA20132830810 |
申请日期 |
2013.10.25 |
申请人 |
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO |
发明人 |
SHIH, STEVE CHAO-CHUNG;BARBULOVIC-NAD, IRENA;WHEELER, AARON |
分类号 |
C12M1/34;C12M1/42;C12M3/04;C12Q1/02;G01N15/06;G01N27/02 |
主分类号 |
C12M1/34 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|