PYRENYLOXYSULFONIC ACID FLUORESCENT AGENTS

摘要

The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.

主权项

1. A compound having the formula:wherein
R and R1′ are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl moieties; X and X1 are independently selected from O, NH or S; s and t are independently selected from the integers from 0 to 3, with the proviso that at least one of s and t is at least 1; R2 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heteroalkyl and substituted or unsubstituted heterocycloalkyl; R3 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl, wherein at least one of R2 and R3 is substituted with a member selected from a reactive group, a moiety comprising a reactive group and a component of a conjugate, and R2 and R3, together with the nitrogen to which they are bound are optionally joined to form a ring which is substituted with a member selected from a reactive group, a moiety comprising a reactive group and a component of a conjugate, with the proviso that, when R2 is H, R3 is a cyclic structure substituted with a member selected from a reactive group, a moiety comprising a reactive group and a component of a conjugate.