摘要 |
The present invention relates to a method for obtaining a mass spectrum of reproducible ions generated at a constant temperature by measuring a total ion count, and the use of a matrix for quantitative analysis using MALDI mass spectrometry. More particularly, the present invention relates to a method for measuring a mass spectrum of ions generated at a constant temperature, the method comprising the step of selecting only mass spectra having the same total ion count, among multiple mass spectra obtained from ions formed by applying energy to a specimen having a sample mixed therein. In addition, the present invention relates to a method for measuring the equilibrium constant of a proton exchange reaction between a matrix and a sample at a constant temperature, a method for obtaining a calibration curve for MALDI quantitative analysis, a method for analyzing the quantity of a sample using the MALDI mass spectrometry, and the use of a matrix for quantitative analysis using the MALDI mass spectrometry. |