METHODS AND COMPOSITIONS FOR HOMOLOGOUS RECOMBINATION IN HUMAN CELLS

摘要

The methods and compositions described herein are based, in part, on the discovery of a stem cell state in human cells that resembles the morphology observed in murine-derived stem cells. Induction of such a state in human stem cells permits an increase in the efficiency of homologous recombination. Thus, the methods and compositions described herein relate to cells and methods for increasing the efficiency of homologous recombination in human stem cells.

主权项

1. A method for generating a genetically-modified human stem cell, the method comprising:
(a) culturing a human stem cell with a GSK3β inhibitor, (b) modifying the genome of the stem cell in step (a) by introducing a homologous recombination cassette and inducing homologous recombination, wherein a genetically-modified human embryonic stem-like cell is generated.