| 摘要 |
The present invention relates to a method of predicting a plant seed trait, such as germination rate, vigour, aging and priming, by determining the presence of a target protein, i.e. diagnostic marker, in its active state in a protein sample derived from a plant seed or plant seed lot. Thereby, the quality of a plant seed or a plant seed lot can be predicted and/or diagnosed and seeds may be distinguished on basis of their characteristics and with respect to the traits described herein. Inter alia, seeds and seed lots having high germination quality may be distinguished from seeds and seed lots having low germination quality. In particular, the method comprises contacting the protein sample or the plant seed or plant seed lot with a chemical probe comprising a warhead being able to attach to an amino acid residue in or nearby an active site of the target protein under conditions allowing the formation of a conjugate of the target protein and the chemical probe, wherein the chemical probe further comprises a reporter tag which is used to detect the conjugate, and wherein detection of the conjugate indicates the presence of the target protein in the protein sample and is used to predict or diagnose the plant seed trait. The present invention further relates to the use of such chemical probes for the prediction of plant seed traits. |
| 主权项 |
1. A method for predicting or diagnosing a plant seed trait by determining presence of a target protein in its active state in a protein sample derived from a plant seed or a plant seed lot, the method comprising:
(i) providing the protein sample; (ii) contacting the protein sample or the plant seed or the plant seed lot with a chemical probe under conditions allowing formation of a conjugate of the target protein and the chemical probe, wherein the chemical probe comprises a warhead capable of attaching to an amino acid residue in or nearby an active site of the target protein, and wherein the chemical probe further comprises a reporter tag and a linker between the warhead and the reporter tag, and (iii) using the reporter tag to detect the conjugate of (ii), wherein detection of the conjugate indicates the presence of the target protein in its active state in the protein sample; and wherein the detection is used to predict or diagnose the plant seed trait, wherein the plant seed trait is selected from the group consisting of germination rate, vigour, priming, seed development, maturation, germination, dormancy, storage deterioration, seed viability, sanitary state, aging and longevity; and wherein the warhead of the chemical probe comprises a reactive group which is selected from the group consisting of epoxides, fluorophosphonases, acyloxymethylketones, epoxyketone, vinyl sulphone, and acylphosphates, or wherein the reporter tag of the chemical probe is a detectable label selected from the group consisting of fluorescent moieties, radioactive moieties, electrochemical moieties, affinity moieties, and reactive moieties. |
