METHOD FOR PRODUCING SECRETABLE ANTIBODIES BY EXPRESSION IN SACCHAROMYCES CEREVISIAE

摘要

The invention relates to a method for the production and non-covalent surface display of antibodies and derived fragments as well as molecule libraries based thereon on the surface of S. cerevisiae cells. The non-covalent manner of the surface display enables specific variants to be selected by means of high-throughput screening and the selected binding molecule to be subsequently controllably secreted into the culture supernatant for biochemical characterization.