PREPARATION OF PARENTAL CELL BANK FROM FOETAL TISSUE

摘要

The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.

主权项

1. An in vitro non-enzymatic method for isolation, expansion and development of foetal cells selected from the group consisting of foetal epiphyseal chondrocytes, foetal Achilles tenocytes or foetal skin fibroblasts, comprising the steps of:
a) using foetal sample selected from ulnar foetal cartilage comprising foetal epiphyseal chondrocyte; foetal Achilles tendon comprising foetal Achilles tenocytes; or foetal abdominal skin comprising foetal skin fibroblasts; b) micro-dissecting and dispersing said ulnar foetal cartilage, foetal Achilles tendon or foetal abdominal skin sample by mechanical attachment to scalpel scored surface; c) culturing said ulnar foetal cartilage, foetal Achilles tendon or foetal abdominal skin sample in vitro under conditions wherein said foetal epiphyseal chondrocytes, foetal Achilles tenocytes or foetal abdominal skin fibroblasts proliferate, d) selecting and isolating first adherent foetal epiphyseal chondrocyte cell populations, first adherent foetal Achilles tenocyte cell populations and first adherent foetal skin fibroblast populations therefrom.