DERIVATIVES OF RECOMBINANT PROTEINS, HOMO-MULTIMERS OF GRANULOCYTE COLONY-STIMULATING FACTOR AND METHOD OF PREPARATION THEREOF

摘要

The invention relates to derivatives of recombinant proteins, comprising homo-multimers of genetically fused recombinant biologically active protein monomer units, connected via selected peptide linker moiety; and the method of preparation thereof. Derivative of recombinant protein is preferably dimer of human granulocyte colony-stimulating factor, characterised by increased circulation time in vivo.

主权项

1. Derivatives of recombinant proteins, comprising homo-multimers of genetically fused recombinant biologically active protein monomer units, connected via suitable peptide linker moiety, wherein each protein monomer unit comprises an amino acid sequence of native biologically active protein or essentially the same sequence of at least 95% identity, said linker moiety being represented by amino acid sequence, selected from the group, consisting of (S-G4)n-S, wherein n=2-7, and SGLEA-(EAAAK)m-ALEA-(EAAAK)m-ALEGS, wherein m=2-8.