| 摘要 |
<p>In accordance with one or more embodiments, the present invention provides pharmaceutical compositions and methods for specific cytotoxic therapy, including, but not limited to, specific non-thermal killing of targeted cells, for example, cancer cells or stem cells, by labeling, binding or introducing into the cytoplasm of the cells, one or more magnetic nanoparticles, such as superparamagnetic iron oxide (SPIO) nanoparticles for example, followed by exposing the cells to oscillating gradients of a magnetic field in the presence of strong static magnetic field such as B0 field generated by modern MRI systems. Specific labeling of cells can be achieved either by various means known in the art, including modifying the cell surface with targeted magnetic nanoparticles (wherein the magnetic nanoparticles are conjugated to one or more antibodies, peptides, or other high affinity ligands), or by loading the cells with nanoparticles internalized by endocytosis, pinocytosis, or through the use of standard cell transfection reagents and techniques. The static magnetic field as well as oscillating gradients can be produced by various known apparatus used to generate such fields, including, standard MRI systems. The methods provided herein make the technology applicable for clinical as well as in vitro use.</p> |
