| 摘要 |
FIELD: biotechnology.SUBSTANCE: for preparing nutrient medium the whole cereal and/or legume grains are used, which are moistened to 15-20%, incubated for 15-30 minutes and subjected to thermal influence in the infrared field for 40-80 sec, maintaining the temperature at the surface of grains within 115-130°C. Hot grain is incubated for 15-30 min, cooled to a temperature of 24-27°C, milled to a particle size of 70-90 microns and the suspension is prepared of the milled grain in water. Enzymatic hydrolysis is carried out of the milled raw material by the exposure of preparations of thermostable alpha-amylase and beta-glucanase for 55-60 min at pH 5.5-6.0 and temperature 65-95°C, and then subjecting the cellulase and cellobiase preparations at 55-60°C to achieve the concentration of reducing substances of 7.5-9.5%. In the fermented suspension mineral salt solution is introduced, heated to 78-80°C, incubated for 10-12 minutes and cooled to 32-33°C. In the resulting nutrient medium the yeast Saccharomyces cerevisiae culture is introduced and the yeast growing is carried out under conditions of intensive aeration to reduce the concentration of reducing substances of 0.3% and less.EFFECT: invention enables to increase yeast biomass yield and protein content in the finished product.3 ex |
