METHOD FOR DETERMINING THE ACTIVITY OF A PROTEOLYTIC ENZYME (VARIANTS), DEVICE FOR THE IMPLEMENTATION OF SAME AND DIAGNOSTIC METHOD

摘要

The invention provides methods for determining the spatiotemporal distribution of activity of a proteolytic enzyme in a heterogeneous system, said method comprising the steps of: (i)providing an in-vitro system, (ii) combining a fluorogenic / chromogeneous / luminescent substrate, (iii) illuminating the sample; (iv) recording the spatial distribution of the signals (v) calculating the spatiotemporal distribution of the proteolytic enzyme activity whereby each step is detailed in the specification and whereby the step of calculating includes correcting the spatiotemporal distribution by using at least one numerical algorithm for reducing noise and distortion of said signal. The methods are useful in medicine and biology and can be used, in particular, for diagnostic and research purposes in order to determine the parameters of blood coagulation system and the components thereof. The invention further provides for devices useful in performing the above methods.