| 摘要 |
Provided is a method for detecting a target particle in a biosample containing pancreatic juice, the method enabling the detection in a solution that has a lower concentration or number density of the target particles than the level possible for conventional photoanalysis techniques. This method comprises: a probe-binding step for preparing a sample solution, which contains a biosample containing pancreatic juice and a fluorescent probe capable of binding to a target particle, and binding the fluorescent probe to the target particle in the biosample; and a calculation step for calculating the number of molecules of the target particles bound to the fluorescent probes by the scanning molecule counting method. A light emission property of emitted light is different between a state where the fluorescent probe is bound to the target particle and a state where the fluorescent probe is present alone. In a state where the fluorescent probe is bound to the target particle, the fluorescent probe emits fluorescence having a wavelength of 600 nm or longer. |
