Compositions comprising eicosapentaenoic acid suitable for high purification

摘要

The invention relates to a microbial biomass composition produced from a heterotrophic fermentation whose fatty acid profile exhibits: an eicosapentaenoic acid (EPA) to arachidonic acid (ARA) ratio of about 11:1 or more; an EPA to total co-concentrating fatty acid ratio of about 8:1 or more, extract compositions, and methods of producing such compositions.

主权项

1. A method of producing a diatom, the method comprising:
a. cultivating diatoms in heterotrophic culture containing a nutrient solution comprising:
(i) a nitrogen source and an organic carbon source at an initial concentration of at least 0.5 M carbon,(ii) a molar ratio of organic carbon to nitrogen of no higher than 30:1 (mol C:mol N), and(iii) a non-limiting source of silicate at a concentration of 150 μM to 1.3 mM at all times during the culture; and b. recovering the diatom from the heterotrophic culture, wherein the cultivating conditions result in production of a diatom having at least 1.5% (w/w) of dry weight as eicosapentaenoic acid and an eicosapentaenoic acid to arachidonic acid ratio of at least 14:1.