| 主权项 |
1. A method of microbial profiling of a sample, comprising:
a. collecting a sample; b. exposing said sample to a phenanthridium derivative of formula (II): wherein R1 is an alkyl having 1 to 6 carbon atoms, perfluoroalkyl having 1 to 6 carbon atoms, or tetra-alkyl ammonium; R2 is a substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or substituted or unsubstituted cycloalkyl having 3 to 10 carbon atoms; R3 is amino, N-alkylamino, N,N-dialkylamino, alkoxy, or carboxyalkyl; and R4 and R5 are independently selected from the group consisting of: hydrogen, halide, hydroxyl, amino, N-alkylamino, N,N-dialkylamino, alkoxy, carboxyalkyl, alkyl having 1 to 6 carbon atoms, perfluoroalkyl having 1 to 6 carbon atoms, azide, and tetra-alkyl ammonium; or a mono or bis halide salt thereof, wherein the phenanthridium derivative has an overall charge of 2+ or higher; c. exposing said phenanthridium derivative containing sample to a light source; d. optionally isolating genomic DNA from said sample; and e. performing PCR on said isolated DNA. |
