Microfluidic platforms for multi-target detection

摘要

Disclosed are example methods and devices for detecting one or more targets. An example method includes placing a sample including a first target with in a microfluidic device and hybridizing a plurality of copies of the first target with a plurality of nanostructures. The example method includes applying an electric current to the plurality of nanostructures and using an electric field created by the electric current to move the plurality of nanostructures. In addition, the plurality of nanostructures are sorted and evaluated to determine at least one of a presence, an absence, or a quantity of the first target.

主权项

1. A method of detecting a plurality of target nucleic acids, the method comprising:
continuously flowing a sample solution comprising the target nucleic acids through a microfluidic device having at least a first surface with a first electrode and a second surface displaced from the first surface at a distance and having a second electrode opposing the first electrode; provided a first and second functionalized nanostructure in solution to the microfluidic device, wherein the first nanostructure is formed by functionalizing a first probe complementary to a first target nucleic acid and the second nanostructure is formed by functionalizing a second probe complementary to a second target nucleic acid; applying an electric current to the electrodes; using the electric field created by the electric current applied to the electrodes to move the nanostructures; trapping the nanostructures within the electric field; mixing the sample solution and the solution providing the nanostructure; hybridizing the target nucleic acids with the nanostructures in the presence of the electric field; hydrodynamically shearing at least one of a non-target or a weakly hybridized target nucleic acid from the nanostructures, wherein the application of the electric current to the nanostructures causes the nanostructure hybridized with the first target to move in a first direction and causes the nanostructure hybridized with the second target to move in a second direction, and wherein the first target and the second target are sorted based on the first direction or the second direction; and evaluating the trapped nanostructures by measuring an electrical impedance between the electrodes to determine a presence, an absence, or a quantity of the first or second target nucleic acids.