Colony-forming unit cell of human chorion and method to obtain and use thereof

摘要

The present invention features colony-forming unit cells derived from the chorion of human placenta and describes compositions and methods for the uses of chorionic cells and their products for therapeutic purposes based upon production and release of multiple growth factors and cytokines by these cells stimulating tissue regeneration independent of engraftment, as well as differentiation into a specific cell type.

主权项

1. Isolated colony-forming unit cells derived from the chorion of human term placentas, wherein the isolated colony-forming unit cells are multipotent stem cells characterized by:
I) expression of protein ACBD6; II) low telomerase activity and ability to be propagated over at least 70 doublings in vitro culture without substantial alteration of karyotype; III) expression of a combination of at least one of the markers from each of: iv) CD-90, SSEA-3, or TRA-1-60; v) a Nanog cytosolic polypeptide; vi) a neurofilament family cytosolic polypeptide; vii) a Nestin cytosolic polypeptide; and viii) one or more secreted polypeptides selected from a family of fibroblast growth factors, hepatocyte growth factors, keratinocyte growth factors, and angiopoietins; and ix) nuclear peptide Oct-4; IV) lack the ability for spontaneous differentiation into lineages of 3 germ layers under standard culture conditions, unless specifically stimulated and lack the ability for formation of teratomas in humans or immuno-compromised animals; V) ability to differentiate into lineages of all 3 germ layers when the cells are exposed to lineage-specific differentiation conditions; VI) ability to grow without adherence to plastic; and VII) inability to form embryoid-like bodies.