Expression vector for animal cell comprising at least one copy of MAR DNA sequences at the 3′ terminal of transcription termination region of a gene and method for the expression of foreign gene using the vector

摘要

Provided are an expression vector for an animal cell including a promoter, a cloning site or a polynucleotide encoding foreign product, and a transcription terminator, all of which are operably connected each other within the expression vector, in which at least one copy of human β-globin MAR sequence is attached to the 31 terminal of the transcription terminator, and a method of expressing a foreign gene using the expression vector.

主权项

1. An expression vector for an animal cell comprising
a promoter, a cloning site or a polynucleotide encoding a foreign gene product, and a transcription terminator, all of which are operably connected to each other within the expression vector, wherein the vector comprises a polynucleotide containing nucleotides 4161 to 10134 of SEQ ID NO:2, in which two copies of human β-globulin MAR sequences are connected adjacently to each other, wherein said two copies of human β-globin matrix attachment region (MAR) are located at the 3′ terminal of the transcription terminator.