J591 minibodies and Cys-diabodies for targeting human prostate specific membrane antigen (PSMA) and methods for their use

摘要

In one embodiment, a minibody monomer that binds PSMA is provided. The minibody monomer is encoded by a nucleotide sequence comprising, from N-terminus to C-terminus, an scFv sequence that can bind PSMA, an artificial hinge sequence, and a human IgG CH3 sequence. In another embodiment, a CysDB monomer that binds PSMA is provided. The CysDB monomer may be encoded by a nucleotide sequence comprising, from N-terminus to C-terminus, an scFv sequence that can bind PSMA and a cysteine tail. In other embodiments, methods for diagnosing or treating a cancer associated with PSMA expression in a subject are provided.

主权项

1. A minibody comprising:
an scFv sequence that can bind prostate specific membrane antigen (PSMA), the scFv comprising a variable heavy domain (VH) linked to a variable light domain (VL) by a linker sequence; an artificial hinge sequence; and a human IgG CH3 sequence, wherein the minibody comprises:
a heavy chain CDR1 that is a CDR1 in SEQ ID NO: 10;a heavy chain CDR2 that is a CDR2 in SEQ ID NO: 10;a heavy chain CDR3 that is a CDR3 in SEQ ID NO: 10;a light chain CDR1 that is a CDR1 in SEQ ID NO: 10;a light chain CDR2 that is a CDR2 in SEQ ID NO: 10; anda light chain CDR3 that is a CDR3 in SEQ ID NO: 10.