Method for methylation analysis of nucleic acid

摘要

The present invention relates to a method for methylation analysis. It comprises the providing of a double stranded nucleic acid; its conversion, whereby unmethylated bases become distinguishable in their base-pairing behavior from methylated bases, and the analysis of both of the converted nucleic acid strands.

主权项

1. A method for methylation analysis of a nucleic acid, comprising
providing a double stranded nucleic acid, converting said nucleic acid in such a way that 5-methylcytosine remains unchanged, while unmethylated cytosine is converted to uracil or to another base that is distinguished by cytosine in its base-pairing behavior, said reaction leading to two different converted nucleic acid strands that are no longer complementary to each other, analyzing both of the converted nucleic acid strands, wherein one of the two strands is analyzed in a methylation specific manner and the other of the two strands is analyzed in a non-methylation specific manner, wherein (i) the methylation-specific manner comprises the analysis of the presence or absence of methylation of one or more cytosines; and (ii) the non-methylation specific manner comprises the analysis of the copy number, deletion or amplification of one or more adjacent nucleotides in a corresponding, overlapping, adjacent or different section of the second strand or an analysis of SNP.