Non-hemolytic LLO fusion proteins and methods of utilizing same

摘要

The present invention provides recombinant proteins or peptides comprising a mutated listeriolysin O (LLO) protein or fragment thereof, comprising a substitution or internal deletion of the cholesterol-binding domain or a portion thereof, fusion proteins or peptides comprising same, nucleotide molecules encoding same, and vaccine vectors comprising or encoding same. The present invention also provides methods of utilizing recombinant proteins, peptides, nucleotide molecules, and vaccine vectors of the present invention to induce an immune response to a peptide of interest.

主权项

1. A recombinant protein comprising a listeriolysin O (LLO) protein comprising a mutation in a cholesterol-binding domain (CBD), wherein said mutation comprises a mutation of residue C484, W491, and W492 of said LLO protein set forth in SEQ ID NO: 37.