| 摘要 |
PURPOSE: A method for preparing a green tea saponin 21-O-angeloyltheasapogenol E3 is provided to massively produce 21-O-angeloyltheasapogenol E3 through hydrolysis using microorganism. CONSTITUTION: A method for preparing 21-O-angeloyltheasapogenol E3 comprises: a step of collecting extract containing saponin from plant seeds using eater or organic solvent; and a step of hydrolyzing the extract using acid, base, enzyme, or microorganism producing the enzyme to isolate the 21-O-angeloyltheasapogenol E3 of chemical formula 1. The plant seed is green tea seed. The acid is hydrochloric acid, sulfuric acid, nitric acid, or ethanol, methanol, or butanol thereof. The base is sodium hydroxide, or potassium hydroxide, or base, ethanol, methanol, or butanol thereof. The enzyme is glucosidase, arabinosidase, rhamnosidase, xylosidase, cellulose, hesperidinase, naringinase,[glucuronidase, pectinase, galactosidase, or amyloglucosidase. |
